2株与霍乱弧菌O139诊断血清发生凝集的麦氏弧菌鉴定

Identification of two Vibrio metschnikovi strains cross-agglutinating with Vibrio cholerae O139

目的对2株疑似O139群霍乱弧菌进一步鉴定,以排除或确诊霍乱。方法以试管凝集和交叉吸收实验复核玻片凝集结果,用生化实验和BIOFOSUN微生物鉴定系统进行生化鉴定,PCR技术检测O139霍乱弧菌特异性脂多糖基因(LPS)。结果玻片凝集阳性,试管凝集1株菌可达原诊断血清效价的1/4,1株菌达1/2,交叉吸收后均证实为非特异性凝集;未检出LPS基因;系统生化鉴定判定为麦氏弧菌。结论2株菌不是O139霍乱弧菌,而是麦氏弧菌。

Objective To identify two strains suspected of Vibrio cholerae O139 to exclude or confirm cholera. Methods Tube agglutination and cross-absorption tests were performed to review the results of slide agglutination. Biochemical experiments featuring BIOFOSUN microbial identification system were conducted, and PCR was applied to detect the Vibrio cholerae O139-specific LPS gene. Results The slide agglutination test was positive. Tube agglutination of one stains demonstrated 1/4 titer of the original serum, and the other 1/2. However, cross-absorption confirmed non-specific agglutination. No Vibrio cholerae O139-specific LPS gene was detected. The biocbemical identification system showed that the strains were Vibrio metschnikovi. Conclusion The two strains were not Vibrio cholerae but Vibrio metschnikovi.