高效液相色谱法测定食品中的牛磺酸

Determination of Taurine in Foods by High Performance Liquid Chromatography

建立了食品中牛磺酸的反相高效液相色谱测定方法。以甲醇－磷酸盐缓冲溶液为流动相（ｐＨ＝４．９），ＺＯＢＡＸＯＤＳ柱为分离柱，紫外检测器（３４０ｎｍ）检测。进样前，先经过磺基水杨酸溶液提取，阴阳离子交换柱净化和邻苯二甲醛（ＯＰＡ）／巯基乙醇衍生化。以谷氨酰氨为内标物，用内标法定量测定。本方法的相对标准偏差＜５％，回收率＞９５％，最小检出限为１２ｎｇ。

Taurine (2 aminoethanesulfonic acid) was quantitated by reversed phase liquid chromatography on a C 18 resolve column (ZOBAX ODS) using a mobile phase of methyl alcohol phosphate buffer (pH=4 9). L glutamine was used as the internal standard. Before separation, the sample was extracted with 0 2 mol/L sulfosalicylic acid solution and cleaned up with dual ion exchange column chromatography. The amino acid was derived with o phthalaldehyde to form an amino acid adduct which was monitored at 340 nm by UV detector. The HPLC method for taurine analysis in foods showed the precision (relative standard deviation) is better than 5%. The recoveries are more than 95% and the detection limit is 12 ng of taurine.