自发性高血压大鼠视网膜自由基损伤的实验研究

An Experimental Study on Free Radical Retinal Damage in the Spontaneously Hypertensive Rats

目的探讨自由基氧化反应在高血压视网膜病变中的损伤作用。方法应用黄嘌呤氧化酶法分别测定１２周、１８周和２４周自发性高血压大鼠（ＳＨＲ）和同周龄正常京都种大鼠（ＷＫＹ）视网膜组织的超氧化物歧化酶（ＳＯＤ）活性；硫代巴比妥酸法分别测定１２周、１８周、２４周ＳＨＲ和同周龄ＷＫＹ大鼠视网膜组织丙二醛（ＭＤＡ）含量。结果各周龄ＳＨＲ视网膜组织中ＳＯＤ活性较同周龄ＷＫＹ减弱，ＭＤＡ含量升高（Ｐ＜０．０５）；不同周龄的ＳＨＲ视网膜组织中ＳＯＤ活性和ＭＤＡ含量差异有显著性（Ｐ＜０．０５）；随ＳＨＲ的周龄增长ＳＯＤ活性减弱，ＭＤＡ含量增高。

Objective\ To explore the role of oxidative reaction of free radicals in the retinal damage after hypertension.\ Methods\ The activities of retinal superoxide dismutases(SOD) and the content of malondialdehyde(MDA) in the retina of 12weekold,18weekold and 24weekold spontaneously hypertensive rat(SHR) and WistarKyoto(WKY) rat were studied.\ Results\ The activities of SOD in retina of SHR reduced,and they gradually reduced as the SHR were elder.\ The content of MDA in the retina of SHR increased,and it gradually increased as the SHR were elder.\ Conclusion\ The oxidative reactions of free radicals participate in the retinal damage after hypertension.