异丙酚对大鼠脑不完全缺血再灌注时脑皮质钙、水代谢的影响

Effects of Propofol on Ca 2+ Overload and Edema Extent in Cortex after Incomplete Cerebral Ischemia and Reperfusion in Rats

目的观察异丙酚对大鼠不完全脑缺血再灌注后脑钙代谢和脑水肿程度的影响。方法ＳＤ大鼠４０只平均分成５组，对照组、缺血组、缺血再灌注组、给异丙酚后缺血组、给异丙酚后缺血再灌注组。以颈动脉夹闭伴低血压（６．６７ｋＰａ）制作不完全性脑缺血，以放开颈动脉夹并使血压恢复至缺血前水平为再灌注。取脑皮质测定脑水和钙含量。结果脑缺血后脑皮质水和钙含量升高（Ｐ＜０．０５），再灌注６０ｍｉｎ后，两指标进一步升高（Ｐ＜０．０１）。异丙酚能明显降低缺血和再灌注后脑钙和水含量升高程度（Ｐ＜０．０５），对脑含水量影响与其对脑钙影响呈正相关（ｒ＝０．９１７，Ｐ＜０．０１）。结论异丙酚能降低不完全脑缺血再灌注后脑皮质钙含量和脑水肿程度，对不完全性脑缺血有一定保护作用。

Objective To observe the effects of propofol on Ca 2+ overload and edema extent of brain cortexes after incomplete cerebral ischemia. Methods 40 Spalgue Dawleg rats were divided into 5 groups:control group;incomplete ischemia 30 min group;infused propofol and incomolete ischemia 30 min group;reperfused 60 min after incomplete ischemia 30 min group;reperfused 60 min after infused propofol and incomplete ischemia 30 min group.\ With clamping bilateral jugular arteries and hypotension(6.67 kPa) to make the incomplete cerebral ischemia.\ Loosening the clamps to reopen the jugular arteries and recovering the arterial blood presure to the level before ischemia to reperfuse the ishemnic brain.\ The cortexes of each rat were taken for measuring the Ca 2+ and water content.\ Results Water and Ca 2+ content in cortexes increased after incomplete cerebral ischemia (P<0.05).\ When reperfused 60 min,water and Ca 2+ content increased further more respectively(P<0.01),propofol was able to prevent from the increasing of water and Ca 2+ in cortexes significantly after incomplete cerebral ischemia respectively.\ Further more the effect of propofol on preventing water increase in cortexes corresponds to its effect on Ca 2+ (r=0.917,P<0.01). Conclusion Propofol has certain protective effect on incomplete cerebral ischemia in rats.\ The mechanism may be related to its effect on preventing the Ca 2+ from overloading in brain cells after brain ischemia.