HMBA诱导胃癌细胞MGc80-3分化过程中NM-LA-IF体系的变化

Changes of NMLAIF System during the Differentiation of MGc803 Cell Induced by Hexamethylene Bisacetamide

目的研究环六亚甲基双乙酰胺（ＨＭＢＡ）对胃癌细胞ＭＧｃ８０－３核骨架－核周层状体－中间纤维（ＮＭ－ＬＡ－ＩＦ）体系的影响。方法使用整装电镜及双向电泳技术观察诱导分化前后ＭＧｃ８０－３细胞ＮＭ－ＬＡ－ＩＦ体系结构及蛋白组成的变化。结果（１）ＨＭＢＡ诱导处理后细胞ＮＭ－ＬＡ－ＩＦ系统结构清晰有序，形成相互作用的骨架网络系统。（２）多肽组成变化显著，除了ＬａｍｉｎａＡ、Ｂ、Ｃ以及两种波形纤维蛋白没有变化外，其余蛋白的含量和种类均有减少。结论该系统结构及多肽组成的差异与胃癌细胞恶性表型逆转有关，且诱导后的细胞分化程度提高。

Objective\ To study the change of nuclear matrixlaminaintermediate filament(NMLAIF) system in MGc803 cell induced by hexamethylene bisacetamide(HMBA).\ Methods\ Using wholemount electron microscopic technique and twodimensional electrophoresis to observe the changes of NMLAIF system in MGc803 cell before and after using HMBA.\ Results\ (1)After induction,the NMLAIF system became a more orderly network.(2)Except Lamina A,B,C and two kinds of vimentin,other polypeptide components diminished or disappeared.\ Conclusion\ The structure and polypeptide components changes of NMLAIF system are relevant to the maligant phenotypic reversion of MGc803 cell and the differentiation state of the cell is higher after induction.