小鼠透明带溶解液诱导人精子顶体反应

Induction of the Acrosome Reaction in Human Spermatozoa by Solubilized Mouse Zona Pellucida

目的研究小鼠透明带溶解液诱导人精子顶体反应的效果，建立人精子顶体反应模型。方法正常生育力人精子经Ｂｉｇｇｅｒｓ，ＷｈｉｔｅｎａｎｄＷｈｉｔｉｎｇｈａｍｍｅｄｉｕｍ（ＢＷＷ）获能５～６ｈ后，分别用小鼠透明带溶解液（ｍＺＰＳ）、ｍＺＰＳ的缓冲液ｈａｌｏｇｅｎｂｕｆｅｒ（ＨＢ）、Ｃａ２＋载体Ａ２３１８７处理１ｈ；对实验和对照组精子用经典的豌豆凝集素（ＰＳＡ）法检测顶体反应。结果ｍＺＰＳ组的顶体反应率高于对照组。结论ｍＺＰＳ能诱导人精子顶体反应，透明带诱导精子顶体反应具有一定的种间交叉性。

Objective\ To investigate the induction of acrosome reaction in human spermatozoa by solubilized mouse zona pellucida(mZPS). \ Methods\ The swimmingup spermatozoa were incubated in Biggers,Whitten and Whittingham medium(BWW) for 5～6 hours at 37℃,and then treated,with mZPS,halogen buffer(the buffer of mZPS),or wellknown acrosome reaction activators calcium ionophose A23187 respectively.\ After an hour.the percentages of acrosomereacted spermatozoa identified by fluoresceinated pisumsativum agglutinin(PSA)and visuallized by fluorescence microscopy.\ Results\ The acrosome reaction rate after spermatozoa treated with mZPS for 1 hour was higher than negative controls. \ Concluslon\ Solubilized mouse zona pellucida are capable of inducing acrosome reaction in human spermatozoa.