急性白血病骨髓基质细胞肿瘤坏死因子α分泌活性及其意义

Secretive activity of tumor necrosis factor α(TNF-α) of marrow stromal cells in acute leukemia and its clinical significance

目的：探讨急性白血病（ＡＬ）骨髓基质细胞肿瘤坏死因子α（ＴＮＦα）分泌活性。方法：应用体外骨髓基质细胞培养法及ＴＮＦα生物活性检测法，对ＡＬ患者２０例及正常对照者１０名的骨髓基质细胞培养上清中的ＴＮＦα活性水平进行检测，并同步检测了患者血清、骨髓白血病细胞培养上清中的ＴＮＦα活性。结果：ＡＬ骨髓基质细胞形成能力较差，但其ＴＮＦα活性明显高于对照组（Ｐ＜０．０５）；少部分急性髓系白血病（ＡＭＬ）骨髓白血病细胞能自发分泌ＴＮＦα；患者血清中的ＴＮＦα活性显著高于正常对照（Ｐ＜０．００１）。结论：ＡＬ患者骨髓基质细胞存在量和质的异常。

Objective:To investigate the TNFα secreting activity of marrow stromal cells in acute leukemia. Methods:TNFα in vitro culture supernate of marrow stromal cells from 20 acute leukemia patients and 10 normal controls was detected. TNFα activities in patients and control serum and in patients leukemic cell culture supernate were also detected. Results: TNFα activity in leukemic stromal cell supernate was significantly higher than that of the control (P<0.05). Some AML cell could spontaneously secret TNFα. Serum TNFα activity of the patient group is also evidently higher than that of the control group (P<0.001).Conclusion: There were qualitative and quantitative defects of marrow stromal cells in acute leukemia patients. Increased secretion of TNFα is probably an important factor leading to the abnormal proliferation of acute leukemia cells.