免疫球蛋白重链胚系基因Cμ在白血病中的异常表达

Aberrant expression of immunoglobulin germline gene Cμ in leukemias

目的：探讨ＩｇＨ胚系Ｃμ基因表达在白血病发病中的意义。方法：应用逆转录聚合酶链反应对各型急、慢性白血病５９例６３份骨髓标本进行免疫球蛋白重链（ＩｇＨ）胚系Ｃμ基因转录本检测。结果：在多种急、慢性白血病亚型中均可以检出胚系Ｃμ链基因的转录。在急性髓系白血病（ＡＭＬ）和急性淋巴细胞白血病（ＡＬＬ）中分别为６５．４％和７５．０％；而慢性粒细胞白血病急变期４例和慢性淋巴细胞白血病２例均呈阳性。ＩｇＨ胚系基因Ｃμ转录本阳性患者中ＨＬＡＤＲ阳性表达者占８０．０％；而在ＡＭＬ伴Ｃμ（＋）者，ＨＬＡＤＲ阳性表达者占９２．９％。对照组均为阴性。结论：相当多的髓系白血病，其白血病细胞系似源于髓系／淋巴系共同干细胞水平，或伴有不可逆性基因重排程序错误及淋巴系。

Objective:To investigate the significance of immunoglobulin germline gene Cμ expression in leukemias.Methods:Immunoglobulin germline gene Cμ transcripts in 63 bone marrow samples from 59 leukemia patients were examined by RT-PCR.Results:Germline Cμ transcripts could be identified in 17 of 26 AML,9 of 12 ALL,4 CMLBC and 2 CLL patients.HLADR was expressed in 80% Cμ(+)patients and in 92.9% Cμ(+) AML patients.In 10 normal bone marrow or peripheral blood samples Cμ transcripts were undetectable.Conclusion:Leukemogenesis in most acute leukemia may originate from the common progenitors of early Blymphoid and myeloid cells.