四例食管癌细胞系的分子细胞遗传学研究

MOLECULAR CYTOGENETIC STUDY ON FOUR HUMAN ESOPHAGEAL CANCER CELL LINES

目的为了探索染色体异常与食管癌的关系。方法应用间期核荧光原位杂交、染色体涂染、比较基因组杂交，对我室建立的４例食管癌细胞系ＥＣ８７１２、ＥＣ８７３３、ＥＣ８５０１和ＥＣ１０９进行分子细胞遗传学研究。结果发现４例细胞系均有１、２、３、８、１６、１７、２０号染色体增加。除ＥＣ１０９源于女性患者外，另３例细胞系均有Ｙ染色体丢失。４例细胞系均有５ｐ扩增，ＥＣ８７３３和ＥＣ８５０１有８ｑ扩增，ＥＣ１０９有８ｑ２４扩增，ＥＣ８７１２、ＥＣ８７３３和ＥＣ８５０１有ｄｅｌ（１）（ｐ）。

Objective To investigate the possible involvement of chromosome abnormalities in pathogenesis of human esophageal cancer.Methods Four cell lines of human esophageal cancer (EC) established in our laboratory were analysed using interphase fluorescence in situ hybridization(FISH), chromosome painting technique and comparative genomic hybridization(CGH).Results Chromosome gain of 1,2,3,8,16,17 and 20 was found in the four cell lines, and loss of chromosome Y in cell line EC8712,EC8733 and EC8501 was noted. Other frequent changes were partial deletion of 1p, translocation of 2q and amplification of 5p in all 4 cell lines, and amplification of 8q and 13q in EC8733 and deletion of 17p in EC8712.Conclusion The data suggest that nonrandom chromosome aberrations may play an important role in the pathogenesis of human esophageal cancer.