摘要
目的为了探索染色体异常与食管癌的关系。方法应用间期核荧光原位杂交、染色体涂染、比较基因组杂交,对我室建立的4例食管癌细胞系EC8712、EC8733、EC8501和EC109进行分子细胞遗传学研究。结果发现4例细胞系均有1、2、3、8、16、17、20号染色体增加。除EC109源于女性患者外,另3例细胞系均有Y染色体丢失。4例细胞系均有5p扩增,EC8733和EC8501有8q扩增,EC109有8q24扩增,EC8712、EC8733和EC8501有del(1)(p)。
Objective To investigate the possible involvement of chromosome abnormalities in pathogenesis of human esophageal cancer.Methods Four cell lines of human esophageal cancer (EC) established in our laboratory were analysed using interphase fluorescence in situ hybridization(FISH), chromosome painting technique and comparative genomic hybridization(CGH).Results Chromosome gain of 1,2,3,8,16,17 and 20 was found in the four cell lines, and loss of chromosome Y in cell line EC8712,EC8733 and EC8501 was noted. Other frequent changes were partial deletion of 1p, translocation of 2q and amplification of 5p in all 4 cell lines, and amplification of 8q and 13q in EC8733 and deletion of 17p in EC8712.Conclusion The data suggest that nonrandom chromosome aberrations may play an important role in the pathogenesis of human esophageal cancer.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
北大核心
1998年第2期75-77,共3页
Chinese Journal of Medical Genetics
基金
攀登计划项目
国家肿瘤攻关课题
关键词
食管肿瘤
细胞系
分子细胞遗传学
CGH
Esophageal cancer Cell lines Chromosome fluorescence in situ hybridization Comparative genomic hybridization