云南省景颇族三个STR位点遗传多态性分析

DISTRIBUTION OF THREE STR LOCI IN JINGPO ETHNIC GROUP IN YUNNAN PROVINCE

目的为了解云南省景颇族三个短串复序列（ＳＴＲ）位点遗传多态性分布。方法应用３个ＳＴＲ位点在同一反应体系中进行互不干扰的复合扩增技术，对云南省景颇族的ＣＳＦ１ＰＯ，ＴＰＯＸ和ＴＨ０１的３个位点等位基因的基因频率进行调查。结果ＣＳＦ１ＰＯ位点，观察到７个等位基因，２６个基因型；ＴＰＯＸ位点，观察到７个等位基因，１９个基因型；ＴＨ０１位点，观察到６个等位基因，１８个基因型。结论获得了满意的结果。

Objective This study was aimed at the use of old bloodstains for investigating the distribution of three STR loci in Jingpo ethnic group.Methods DNA extraction from old bloodstains (106 in number) and multiplex amplification of CSF1PO, TPOX and TH01 were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of CSF1PO, TPOX and TH01 loci in a Jingpo ethnic group in the southwestern part of Yunnan province.Results 7 alleles and 26 genotypes of CSF1PO locus, 7 allesles and 19 genotypes of TPOX locus, and 6 alleles and 18 genotypes of TH01 locus were observed. Conclusion The satisfactory results demonstrate that multiplex amplification of CSF1PO, TPOX and TH01 is sensitive and the old stain of a drop of blood is sufficient for such amplification.