急性缺氧对血管内皮细胞活力和凋亡的影响

Effects of acute hypoxia on vascular endothelial cell activity and apoptosis

目的研究急性缺氧对血管内皮细胞活力和凋亡的影响。方法人脐静脉血管内皮细胞，缺氧（5％O2）条件下培养0、1、3、12h，四甲基偶氮唑盐比色法（MTT法）检测细胞活力，膜联蛋白荧光素法（Annexin V-Fluos）检测相应时间细胞凋亡率。结果急性缺氧早期，血管内皮细胞的活力增加，缺氧3h达顶峰，之后随缺氧时间延长有所降低，至缺氧12h低于正常。凋亡检测结果显示，缺氧3h即能引起内皮细胞凋亡，12h凋亡进一步加重。结论急性缺氧早期细胞活力及凋亡均增强，提示细胞适应性反应与损伤性反应并存，这种适应性反应将有助于细胞对抗缺氧造成的损伤。

Objective To explore effects of acute hypoxia on vascular endothelial ceil activity and apoptosis. Methods Human vascular endothelial cell line （ECV304） was cultured in 5% CO2 incubator for 0 h,1 h,3 h,12 h. MTT method was used to detect the activity of the cultured cells, and Annexin V-Fluos method was applied to detect the apoptotic rate of the cells in restricted time. Results At the early stage of hypoxia, endothelial activity enhanced, reached the peaked level at 3 h after hypoxia, then decreased thereafter with the duration of hypoxia, but was still lower than the normal level. Apoptotic analysis revealed that 3 h hypoxia could induce endothelial apoptosis, with the apoptotic rate increasing to （ 22.64 ± 0.63 ） % , then apoptosis decreased at 12 h following hypoxia. Conclusions At the early stage of hypoxia, both cell activity and apoptosis of endothelial cells enhanced following acute hypoxia, indicating that adaptive and traumatic responses co-existed in the endothelial cells. This adaptive response would help cells to resist injuries induced by hypoxia.