人类白细胞抗原-1类抗原B7基因cDNA克隆

cDNA Cloning of HLA-B7 Gene

采用逆转录 PCR（聚合酶链反应）技术,从人类白细胞抗原-B7（HLA-B7）表型阳性的人外周血淋巴细胞总RNA中克隆了HLA-B7的cDNA（pUC-B7）。经过PCR扩增和酶切片段长度多态性分析,初步判断其序列与文献报道的一致。采用平端连接将 HLA-B7 cDNA片段插入至 pUC19的Sma 1位点上,保留了大部分的酶切位点。为进一步克隆到表达载体上提供了极大的方便。

HLA-B7 cDNA was cloned by reverse transcription PCR from human peripheral blood lymphocytes. The cDNA fragment was identified by restriction enzyme digestion and PCR detection. The results corresponded to the data from Ashwaii K et al. In order to keep most of the enzyme sites, HLA-B7 cDNA was inserted to Sma I site of pUC19 by blunt Unking. It provided great convenience for constructing expression vector in the further study.