镉及茶多酚对大鼠心肌细胞动作电位的影响

Effect of cadmium and tea polyphenols on action potential in rat myocardial cells

目的:探讨氯化镉(CdCl2)及茶多酚(TP)对大鼠心室肌细胞动作电位(AP)的影响.方法:用常规微电极方法记录心室肌细胞AP.结果:不同浓度的CdCl2可使心肌细胞动作电位幅度(APA)值降低,最大除极速率(Vmax)降低,复极化时程(APD)明显缩短.CdCl20.005mmol/L组APA从74.9±4.8降至66.5±6.7;Vmax从15.3±2.3降至16.1±2.5;CdCl20.01mmol/LAPA降至50.3±2.4;Vmax降至9.3±0.9.0.015mmol/LAPA降至46.2±3.1;Vmax降至3.7±0.6.结论:CdCl2能够抑制心肌细胞的AP,TP可减轻镉对心肌细胞AP的影响.

AIM： To investigate the effect of CdCl2 and tea phlyphenols（TP） on the action potential in rat myocardial cells. METHODS： Action potentials were recorded using standard microelectrode technique. RESULTS： CdCl2 of different concentrations decreased the amplitude of action potential（APA） and the maximum rate of depolarization （Vmax） , and shortened the action potential duration of repolarization （APD）. CdCl2/L at 0. 005 mmol decreased APA from 74.9±4.8 to 66.5±6.7 and Vmax from 15.3±2.3 to 16.1 ±2.5, CdCl2/L at 0.01 mmol decreased APA to 50.3±2.4 and Vmax to 9.3±0.9 and CdCl2/L at 0. 015 mmol decreased APA to 46.2±3.1 and Vmax to 3.7 ±0.6. TP decreased the effects of CdCl2 on the action potential in rat myocardium. CONCLUSION： CdCl2 inhibits the action in rat myocardial cells and TP decreases the effects of CdCl2 on the action potential in rat myocardium.