水热法制备细胞标记用CdSe半导体量子点材料(英文)

HYDROTHERMAL SYNTHESIS OF SEMICONDUCTOR CdSe QUANTUM DOTS MATERIAL FOR CELL MARKING

介绍了水热合成CdSe半导体纳米晶的工艺,调节反应温度及时间有助于加速CdSe纳米晶的生长,使其荧光产率得到显著提高;在一定的初始条件下,随着纳米晶核长大,产物的荧光产率单调增至极值然后逐渐下降;CdSe荧光产率的最大值与反应温度关系密切。实验利用1-乙基–1-3-(3-二甲基氨基-丙基)–碳化二亚胺将所制备的CdSe与转铁蛋白偶联成生物探针,成功标记了人肺腺癌细胞。

The hydrothermal route to the synthesis of CdSe semiconductor nanocrystals was presented. With the adjusting of the reaction temperature and time,the growth rate of the CdSe nanocrystals is accelerated and their photoluminescence quantum yield（PLQY） is improved remarkably. During the growth,the PLQY of CdSe nanocrystals under a given set of initial conditions increases monotonically to the maximum value and then decreases gradually. The highest PLQY is strongly dependent on the growth temperature. Using 1-（3-dimethylaminopropyl）-3-ethylcarbodiimide hydrochloride as coupling agent, as-prepared CdSe quantum dots are conjugated with Transferrin to form the bioprobe and human lung adenocarcinoma cells are successfully marked.