KIR在NK-92MI及K562细胞中的基因型及表达谱分析

Killer immunoglobulin-like receptor gene genotype and expression pattern in NK-92MI and K562 cells

目的:分析杀伤细胞免疫球蛋白样受体(KIR)在NK-92MI及K562细胞中的基因型及表达谱,探索KIR受体的表达调控规律。方法:采用PCR、RT-PCR及分子克隆测序技术,检测K562及NK-92MI细胞中KIR2DL1、2DL2、2DL3、2DL4、2DL5、2DS1、2DS2、2DS3、2DS4、2DS5、3DL1、3DL2、3DL3、3DS1、2DP1、3DP1的基因型,及KIR2DL1、KIR2DL2、KIR2DL3、KIR3DL1、KIR2DS1、KIR2DS3及KIR2DS2/4受体mRNA的表达。结果:K562细胞携带KIR2DL1、2DL2、2DL3、2DL4、2DL5A、2DS2、2DS4*003-006、2DS5、3DL1、3DL2、3DL3基因,但不表达KIR。NK-92MI细胞携带KIR2DL1、2DL2、2DL3、2DL4、2DS2、2DS4*001/002、2DS4*003-006、3DL1、3DL2、3DL3,但只表达KIR2DL1、KIR2DL3及KIR2DS2/4。结论:K562细胞及NK-92MI细胞均携带KIR2DL1、2DL2、2DL3、2DL4、2DS2、2DS4*003-006、3DL1、3DL2、3DL3。NK-92MI细胞表达KIR受体,K562细胞不表达上述KIR,KIR呈细胞特异性表达。

AIM：To make clear the repertoire of Killer immunoglobulin-like receptor at the level of DNA and RNA in NK-92MI and K562 cells and compare KIRs gene genotype and expression patterns in both cells. METHODS：KIRs gene genotype,include KIR2DL1,2DL2,2DL3,2DL4,2DL5,2DS1,2DS2,2DS3,2DS4,2DS5,3DL1,3DL2,3DL3,3DS1,2DP1,3DP1,was analyzed by PCR. KIRs gene phenotype,include KIR2DL1,KIR2DL2,KIR2DL3,KIR3DL1,KIR2DS1,KIR2DS3,KIR2DS2/4 gene,was determined by RT-PCR and sequencing. RESULTS：KIR2DL1,2DL2,2DL3,2DL4,2DL5A,2DS2,2DS4＊003-006, 2DS5, 3DL1, 3DL2 and 3DL3 gene were present in t（562 cells, but all corresponding receptors were not expressed by K562 cells. KIR2DL], 2DL2, 2DL3, 2DL4, 2DS2, 2DS4 ＊001/002, 2DS4 ＊ 003-006, 3DL1, 3DL2 and 3DL3 gene were present in NK-92MI cells, but only transcripts of KIR2DL1, KIR2DL3 and KIR2DS2/4 were detectable. CONCLUSION： KIR2DL1, 2DL2, 2DL3, 2DL4, 2DS2, 2DS4 ＊ 003-006, 3DL1, 3DL2 and 3DL3 gene were present in both 14562 and NK-92MI cells. All corresponding KIR receptors were not expressed by K562 cells and only partial KIRs by NK-92MI cells. The repertoire of KIRs were specific for K562 and NK-92MI cells.