成神经分化的骨髓间充质干细胞向C6细胞条件培养基和SDF-1α的趋化性迁移研究

Tropism of bone marrow mesenchymal stem cells in neurogenic differentiation for C6 conditioned medium and SDF-1α

目的:探讨成神经分化的骨髓间充质干细胞(BM-SCs)向C6细胞条件培养基和SDF-1α的趋化性迁移。方法:采用碱性成纤维生长因子(bFGF)、丁羟基茴香醚(BHA)、二甲基亚砜(DMSO)联合诱导剂对BMSCs进行诱导分化,通过免疫荧光染色检测诱导的细胞表达神经前体细胞标志物Nes-tin、β-III-Tubulin和NSE的情况。运用Dunn chamber研究成神经分化的BMSCs向胶质瘤和SDF-1α的迁移。结果:BM-SCs能诱导分化成神经元样细胞,而对照组的BMSCs形态无变化。Dunn chamber分析显示,C6细胞条件培养基组和SDF-1α组诱导细胞的迁移速率和迁移效率明显高于对照组,表明C6细胞条件培养基和SDF-1α对BMSCs具有趋化作用,单个细胞迁移轨迹实验也证实了这一结果。此外,分化不同状态的BMSCs向C6细胞条件培养基和SDF-1α的趋化程度也不同。结论:BMSCs的定向迁移与分化状态密切相关。

AIM：To investigate the tropism of bone marrow mesenchymal stem cells （BMSCs） in neurogenic differentiation for glioma and SDF-1α. METHODS：BHA,bFGF,and DMSO were used to induce the neural differentiation of BMSCs,and the expression of neural markers,Nestin,β-III-Tubulin and NSE were analyzed by immunocytochemistry. We investigated,by using the Dunn chamber,the migration of BMSCs in neurogenic differentiation towards glioma and SDF-1α. RESULTS：BMSCs could be induced to differentiate into neuron-like cells, while the control remained the morphology of BMSCs. Dunn chamber analysis revealed that both the migration speed and efficiency of cells induced by C,6 conditioned medium and SDF-1α were higher than control, demonstrating the chemotactic effect of both C6 conditioned medium and SDF-1α on BMSCs. These results were confimed by examination of migration tracks of in- dividual cells. Moreover, BMSCs at different states of differentiation showed different degree of tropism for C6 condi- tioned medium and SDF-1α. CONCLUSION： The directed migration of BMSCs is closely related to the differentiation states of these cells.