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急性髓性白血病患者异基因造血干细胞移植后早期T细胞免疫重建的研究 被引量：1

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摘要 目的:监测急性髓性白血病(AML)患者接受异基因造血干细胞移植(allo-HSCT)后外周血中的T细胞受体重排删除环(TRECs)水平,了解移植后早期的T细胞免疫重建情况。方法:AML患者15例分别在allo-HSCT前以及allo-HSCT后每隔2周收集外周血,采用实时定量PCR法检测外周血单个核细胞(PBMNCs)中的TRECs水平;7例年龄相近的正常人外周血样本作为对照。流式检测CD45RA+细胞亚群和CD45RO+细胞亚群的阳性率。结果:allo-HSCT组移植前的TRECs平均拷贝数为(1.42±1.51)拷贝/1000 PBMNCs,明显低于正常组的(3.03±0.45)拷贝/1000 PBMNCs水平;移植后第12周的TRECs平均拷贝数为(1.67±1.93)拷贝/1000 PBMNCs,与正常相比仍然较低。移植后8周内以CD45RO+/CD4+细胞的扩增为主,而外周血TRECs水平在allo-HSCT后4周左右出现短暂地升高趋势,随后又下降,至allo-HSCT8周后才开始稳步上升。有急性移植物抗宿主病(aGVHD)病史的移植患者的外周血TRECs水平显著降低,明显低于无aGVHD病史移植患者的外周血TRECs水平。3例移植后早期复发的患者的外周血TRECs水平降至基线水平或检测不到。结论:不同个体T细胞免疫重建时间存在差异;AML患者在allo-HSCT后早期(90d内)的胸腺输出功能仍处于恢复阶段,移植后4周内的免疫重建仍以来源于供者细胞的外周扩增为主;外周血TRECs水平可能与AML患者allo-HSCT后病情的发展、转归有关。
作者 吴秀丽 李扬秋 朱康儿 杨力建 陈少华 卢育洪 陈洁 刘启发
机构地区 南方医科大学南方医院血液科 暨南大学医学院血液病研究所 暨南大学附属一院血液科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第9期828-830,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30270579) 广东省自然科学基金重点项目(23001) 广东省教育厅"千百十工程"优秀人才培养基金项目(Q校02022) 中国博士后科学基金资助项目(20080440776)
关键词 造血干细胞移植 异基因 T细胞受体重排删除环 免疫重建
分类号 R318.6 [医药卫生—生物医学工程]
  • 相关文献

参考文献8

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二级参考文献31

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共引文献27

同被引文献16

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细胞与分子免疫学杂志
2009年 第9期

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