C6鼠胶质瘤模型及表皮生长因子受体反义RNA治疗的MRI动态观察

MR imaging in rat glioma model before and after EGFR antisence RNA therapy

目的用１．５ＴＭＲ机动态观察Ｃ６鼠胶质瘤的变化及表皮生长因子受体（ＥＧＦＲ）反义ＲＮＡ对其治疗的价值。材料与方法将４３只体重１５０～２００克雄性Ｗｉｓｔａｒ大鼠分４组：Ａ组为正常组，７只；Ｂ组为右尾状核接种Ｃ６细胞组，１６只；Ｃ组为在接种的胶质瘤内注射ＥＧＦＲ反义ＲＮＡ组，１２只；Ｄ组为右尾状核接种经ＥＧＦＲ反义ＲＮＡ转染的Ｃ６细胞组，８只。Ａ组行ＭＲ平扫及增强扫描；Ｂ～Ｄ组动态观察ＭＲ平扫和增强扫描的改变，并于各时间点处死大鼠，取脑行病理检查。结果ＭＲＩ可清晰显示正常大鼠大脑半球等结构。接种后１周即可显示瘤灶。ＭＲＩ动态观察可判断Ｃ６鼠胶质瘤的变化，Ｃ、Ｄ组的肿瘤生长比Ｂ组明显受到抑制且与病理一致。结论１．５ＴＭＲ机可清晰显示Ｃ６鼠胶质瘤自然过程及治疗后的变化；

Purpose To study the value of 1.5T MRI in long term follow up of rat C6 glioma model and the efficacy of EGFR antisence RNA therapy in vivo. Materials and methods Forty three male Wistar rats, weighing 150 to 200g, were used for this study. They were divided into four groups: group Ⅰ: 7 normal rats; group Ⅱ: 16 rats inoculated with C6 cells in right caudate nucleus; group Ⅲ: 12 rats injected with EGFR antisence RNA in the inoculated tumors; group Ⅳ: 8 rats inoculated with C6 cells transfected by EGFR antisence RNA in right caudate nucleus. Group Ⅰ was examined by plain and enhancement MR scanning. Group Ⅱ～Ⅳ were followed up by plain MR scanning and enhancement MR scanning and were sacrificed in various time points for pathological examination. Results The hemispheres of normal rat could be seen clearly in the MR imaging. The tumor could be seen about 1 week after inoculation. According to findings on the follow up MR scan, growth of the tumor or its regression after treatment were clearly observable. The tumor growth was significantly inhibited in group Ⅲ and group Ⅳ as compared to those in group Ⅱ. Conclusion The growth of the rat C6 glioma model and its change after treatment could be shown clearly on 1.5T MR imaging; EGFR antisence RNA significantly inhibited the growth of glioma in vivo.