摘要
目的对2003年12月、2006年6月和2008年6月3次采集的严重急性呼吸综合征(severe acute respiratory syndrome,SARS)患者血清进行冠状病毒抗体(SARS-CoVIgG/IgM)的动态检测,并分析其意义。方法选择2003年确诊的21例SARS患者为研究对象,同期工作的29例未感染人群为对照组,采用酶联免疫法(ELISA)检测抗SARS-CoVIgG和IgM抗体,采用GEN-S全自动血球分析仪器对患者血细胞进行分析,采用AU5400生化分析仪测定血清免疫球蛋白含量变化。结果SARS患者在感染后第3年血清抗SARS-CoVIgM抗体阳性率为23.8%(5/21),第5年抗SARS-CoVIgM全部消失;而抗SARS-CoVIgG抗体在感染后第3年其滴度有下降趋势,但全部为阳性,感染后第5年血清抗SARS-CoVIgG阳性率高达90%(19/21)。对照组血清抗SARS-CoVIgM/IgG全部阴性。所有感染者在感染后第3年和第5年血液细胞分类和血清免疫球蛋白含量均与正常人群比较无显著差异。结论SARS患者在感染冠状病毒后第5年表现为抗SARS-CoVIgG持续阳性,其血细胞分类和血清免疫球蛋白等指标持续正常,支持SARS-CoVIgG可做为保护性抗体的说法。
Objective To investigate the changes of anti-SARS-CoV IgG and IgM antibodies in the sera of SARS patients collected in December 2003, June 2006 and June 2008 and evaluate its significance. Methods Twenty-one subjects diagnosed as SARS in 2003 and 29 healthy controls were included in the study. Enzyme-linked immunosorbent assay （ELISA） was applied in detecting anti-SARS-CoV IgG and IgM antibodies in 63 serum samples of 21 SARS patients. Hematocyte counts were determined by GEN-S analyzer and the levels of immune globulins were measured by AU5400. Results Anti-SARS-CoV IgM antibody was detected in 23.8% of SARS patients in the 3rd year of SARS infection and was undetectable in the 5th year, All the SARS patients were positive for anti-SARS-CoV IgG antibody in the 3rd year of SARS infection, with a little decline in the titers, and the positive rate was as high as 90% in the 5th year. All the healthy controls were negative for anti-SARS-CoV IgG and IgM antibodies. The differences in hematocyte counts and the levels of immune globulins were not significant between the two groups in the 3rd and the 5th year. Conclusions Anti-SARS-CoV IgG antibody is persistantly present in the sera of SARS patients in the 5th year, with normal hematocyte counts and normal levels of immune globulins. The results indicate that anti-SARS-CoV IgG antibody may be the protective antibody against SARS infection.
出处
《传染病信息》
2009年第4期235-237,共3页
Infectious Disease Information
