替米考星对内毒素炎症小鼠存活率和细胞因子的影响

Effects of tilmicosin on cytokine response and survival rate of inflammatory mice by LPS

替米考星按20 mg/kg剂量经灌胃给药后,观察其对内毒素(LPS)炎症小鼠的保护率,并通过不同时间段眼球采血,分离血清,应用试剂盒用酶联免疫吸附分析(Enzyme-linked i mmunosorbent assy,ELISA)法测定小鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、白细胞介素-6(IL-6)、白细胞介素-1(IL-1β)的水平。结果表明,替米考星明显提高了内毒素炎症小鼠的存活率,死亡率由100%降低为50%(P<0.05)。LPS(20 mg/kg)能提高血清中TNF-α、IL-10、IL-6和IL-1β的水平,替米考星能够显著抑制TNF-α、IL-6和IL-1β的释放,而明显提高IL-10的水平。

The inflammation mice model was established by intraperitoneal injection of LPS（20 mg/kg）,and administered the tilmicosin （20 mg/kg） by gavage. The results showed the tilmieosin remarkably improved the survival rate of mice challenged with LPS. Compared with LPS group at 24 hour,the mortality of timilcosin group obviously dropped from 100% to 50% （P〈0.05）. There was a distinct decrease in serou TNF -α,IL-10,IL -6 and IL-1β contents and increase in serou IL- 10 levels in tilmicosin group compared with LPS group.