黑脊倒刺鲃原生殖细胞起源和迁移的研究

Origin and Migration of PGCs in Spinibarbus caldwelli

在显微及亚显微的水平上,对黑脊倒刺鲃原生殖细胞(PGCs)的起源和迁移的过程进行了研究.PGCs最早发现于低囊胚期的囊胚层,后来从早期胚胎的下胚层迁移到脏壁中胚层,再沿着肠系膜迁移到两侧的生殖嵴中,最终与生殖嵴细胞共同组成未分化的性腺.其迁移过程既与周围组织细胞的推动有关,也与其自身的主动迁移能力有关.

Origin and migration of PGCs （primordial germ cells）from a teleost, Spinibarbus caldwelli were studied at microscopic and submicroscopic levels. PGCs were associated with the blastoderm margin at first and subsequently remained in the hypoblast of the em- bryonic shield region during mid-gastrula and neurula stage. Later migrated to the splanchnic mesoderm during the stage of muscular effect and then proceeded around the gut to the dorsal mesentery during hatching stage. In the end, PGCS translocated to the genital ridges and formed the indifferent gonad with the somatic cells of genital ridges. The whole migration process had something to do with both the surrounding tissues and the active migration ability of PGCs themselves.