鳡遗传多样性的ISSR分析

Analysis of Genetic Diversity of Yellowcheek Carp Elopichthys bambusa by ISSR Markers

利用ISSR分子标记技术对长江下游苏州段野生和野生F1代人工养殖的2个鳡群体遗传多样性进行分析研究。从77个ISSR引物中筛选出4个引物对鳡2个群体48个样品进行扩增,得到41个清晰的扩增位点。鳡野生和野生F1代人工养殖2个群体的多态位点比率和群体内遗传多样性指数分别为21.95%、17.07%,0.0724、0.0426;前者遗传多样性较后者略高。基因分化系数Gst和群体内遗传多样性指数估算分析均显示2个鳡群体之间出现一定遗传分化。鳡UPGMA系统树有较明显的歧化,表现出一定的遗传趋异。结果分析表明,鳡群体的遗传多样性相对贫乏;野生F1代人工养殖群体尚未形成自己独立的遗传结构,但2个群体间已经产生了一定的遗传分化,经过较多世代的人工繁育有可能形成自己独立而稳定的遗传结构。

Genetic diversity of a wild population collected from Suzhou area in Yangtze River and a cultured population from hatchery-reared offspring of the wild caught from the same area of yellowcheek carp （Elopichthys barnbusa） was investigated using Inter-simple sequence repeat （ISSR）. Four primers produced 10 polymorphic markers out of the total of 41 bands amplified from 48 total individuals. The proportions of polymorphic loci of the wild population and the cultured population were 21. 95% and 17.07 %, respectively. These Shannon＇s Information Index and average heterozygosity were 0. 0724 and 0. 0426,0. 454 and 0. 245, respectively. The results showed that there was a little differentiation in the genetic diversity of these between the two populations. Gst value and Shannon＇s Information Index indicated that there was some genetic differentiation between the two populations. The UPGMA tree based on ISSR data supported the results. The ISSR analysis indicated that the two populations had lower genetic diversity, and the cultured population formed own stable genetic structure by multi-generation artificial breed- ing.