通心络胶囊对载脂蛋白E基因敲除小鼠冠状动脉粥样硬化的影响

Influence of Tongxinluo capsule on coronary atherosclerosis of Apolipoprotein E gene knockout mice

目的:通过通心络胶囊对载脂蛋白E基因敲除小鼠[ApoE(-/-)]冠状动脉斑块和心肌病理组织学的干预变化,探究其治疗冠心病(CHD)动脉粥样硬化(AS)的疗效机制。方法:将40只ApoE(-/-)小鼠作为实验组,建立冠状AS模型,随机分为模型组和辛伐他汀组等4组,后者分别给予辛伐他汀及高、中、低剂量的通心络胶囊进行干预8周;另选8只同系的小鼠作为正常对照组。通过观察各组小鼠冠状动脉斑块和心肌病理组织学等指标的变化,探讨通心络胶囊对ApoE(-/-)小鼠AS的调节作用和机制。结果:模型组小鼠冠状动脉病变主要位于心肌内的小分支、心肌细胞、心肌间质,均分为6.63±1.48;辛伐他汀组和通心络胶囊各剂量组冠状动脉及心肌病变较模型组均减轻,其中辛伐他汀组均分为2.62±2.25,通心络胶囊低剂量组均分为4.5±1.71,中剂量组均分为3.12±1.81,高剂量组均分为2.38±2.34。通心络胶囊低剂量组与模型组比较,P<0.05;通心络胶囊中、高剂量组与模型组比较,均为P<0.01;辛伐他汀组与模型组比较,P<0.01;通心络胶囊中、高剂量组与辛伐他汀组比较,P>0.05。结论:通心络胶囊对冠状AS的形成有一定的抑制作用,各剂量组作用强度依次为高、中、低剂量组,其中中、高剂量组与辛伐他汀组作用相当。

Objective：To explore the therapeutic mechanism of Tongxinluo capsule treating atherosclerosis in coronary heart disease by measuring the changes of coronary plaques and myocardial histopathology of Apolipoprotein E gene knockout mice[ApoE （-/-）] intervented by Tongxinluo capsule. Methods：Forty Apolipoprotein E gene knockout mice [ApoE （-/-）]were taken as the experimental group,to establish the models of coronary atherosclerosis,then randomly divided to 5 groups（Model group ,Simvastatin group and Tongxinluo group）,the laters were intervented for 8 weeks by Simvastatin and Tongxinluo capsule with high dose,middle dose and low dose respectively,while other 8 isogeneic mice as the normal control group. The effect and potential mechanism of Tongxinluo capsule on atherosclerosis in [ ApoE （-/-）] mice were researched by observating the changes of some indexes in each group, such as coronary plaques and myocardial histopathology, and so on. Results ：The lesions of coronary in mice of model group were mostly located at small brancha in cardiac muscle,cardiac muscle cell,myocardial interstitium,and the average score is 6.63±1.48. Compared with model group, Simvastatin group and different dosage of Tongxinluo capsule groups had less coronary and cardiac muscle lessions, the average score of the Simvastatin group was 2.62±2.25 （P 〈 0.01）, the low dose of Tongxinluo capsule was 4.5±1.71 （P 〈 0.05）, the middle dose group was 3.12±1.81（P 〈 0.01）,and the high dose group was 2.38±2.34（P 〈 0.01）. There were no statistically significant differences between Simvastatin group and high-and middle-dose Tongxinluo group（P 〉 0.05）. Conclusion：Tongxinluo capsule had significant inhibition effect in coronary atherosclerosis formation in the Simvastatin group compared with high-and middle-dose Tongxinluo group in adose-dependent model. While the high-dose and middle-dose Tongxinluo had the similar effect with Simvastatin group.