ABCG8 T400K基因多态性与高脂血症的关系

Interactions between ATP binding cassette G8 T400K polymorphisms and hyperlipidemia.

目的探讨ABCG8 T400K基因多态性与高血脂症的相关性。方法采用酚-氯仿法提取370例高脂血症患者及303例健康志愿者DNA,用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术检测ABCG8 T400K基因多态性,用χ2检验检测基因多态性与高脂血症的关系。结果ABCG8 T400K基因多态性与高脂血症患病风险间显著相关,ABCG8 400K等位基因为患高脂血症的高风险因素(OR=1.870,P=0.002)。结论ABCG8 T400K基因多态性与高脂血症患病风险间显著相关,ABCG8 400K等位基因携带者患高脂血症的风险较高。

Objective To investigate the effects of ABCG8 T400K gene polymorphisms on hyperlipidemia. Methods DNA of 303 healthy controls and the 370 hyperlipidemic subjects was extracted from peripheral blood with the method of phenol - chloroform. ABCG8 T400K polymorphism was screened by polymerase chain reaction - restriction fragment length polymovphism（PCR - RFLP）. Chi -square test was used to analyzed interactions between ABCG8 T400K polymorphism and hyperlipidemia. Results There was great relationship between the polymorphism of ABCG8 T400K and hyperlipidemia, and the allele of ABCG8 400K predicted the hyperlipidemic events（OR=1.870, P = 0.002）. Conclusion There was great relationship between the polymorphism of ABCG8 T400K and hyperlipidemia, there was higher risks in the subjects with allele of ABCG8 400K.