睾酮与雄激素受体在中国林蛙精巢中的免疫细胞化学定位

Immunocytochemical localization of testosterone and androgen receptor in testis of Rana chensinensis

用免疫细胞化学方法检测了睾酮(T)和雄激素受体(AR)在中国林蛙生精周期中不同时期精巢内的表达定位.结果显示:在中国林蛙生精周期的Ⅰ～Ⅴ期,T和AR在精原细胞、精母细胞、精子细胞、精子、支持细胞和间质细胞内均有分布.在不同时期的精巢中,T和AR在生精细胞的定位具有一致性.在Ⅱ期,精子细胞内T阳性反应和AR阳性表达最强.在生精周期的Ⅰ～Ⅴ期,支持细胞内T的阳性反应强度无明显变化.在Ⅴ期,支持细胞内AR的阳性表达强度显著高于其他各期,而间质细胞内AR的阳性表达最弱.结果表明,T在中国林蛙精巢内细胞的分化和发育过程中的作用具有高度可变性,T调控中国林蛙精巢的功能和发育.

Localisation of testosterone（T）and androgen receptors（AR）was investigated in different stages of the spermatogenetic cycle of Rana chensinensis,using immunocytochemistry.The results showed that both T and AR are distributed in spermatogonia,spermatocytes,spermatids,spermatozoa,Sertoli and Leydig cells from stage Ⅰ to stage Ⅴ of spermatogenesis.A strong positive distribution of T and AR was observed in spermatids in stage Ⅱ.Expression of T in Sertoli cells no significantly changes in intensity from stage Ⅰ to stage Ⅴ of spermatogenesis.In stage Ⅴ,expression of AR in Sertoli cells was higher than that in other stages.Nevertheless,expression of AR in Leydig cells was lower in stage Ⅲ than that in other stages.The results suggested that the roles of T in the differentiation and development of the spermatic cells were highly diverse.There is a T regulation of the function and development of the frog testis.