HPLC法测定泌淋清片中盐酸小檗碱的含量

Determination of Hydrochloride Berberine in Milinqing Tablets by HPLC

目的:建立泌淋清片中盐酸小檗碱的含量测定方法。方法:采用高效液相色谱法,以Hypersil ODS2柱(C18,4.6×250mm,5μm)为分离柱,以乙腈:0.05mol/L三乙胺溶液(29:71)为流动相,345nm为检测波长,柱温为室温。结果:盐酸小檗碱在10.2～61.2μg/mL范围内,峰面积与其浓度呈良好线性关系(r=0.9993),平均回收率为97.5%,RSD%为0.34%,n=5)。结论:此法简便、准确、重复性好,可作为该制剂含量测定方法。

OBJECYIVE： Aim to set up determination the contents of Hydrochloride Berberine in Milinqing Tablets.METHODS：HPLC method was used.Chromatographic condition included Hypersil ODS2 （C 18, 4.6×250mm, 5μm） and the mobile phase consisting of acetonitrile-0.05mol/1 triethylamine solution （29：71） ,with a detection wavelength of 345nm and the temperature to room temperature.RESULT：The calibration curves were linear in the range of 10.2-61.2μg/mL for Hydrochloride Berberine, r=0.9993, average recovery of Hydrochloride Berberine Was 97.5%,RSD Was 0.34% （n=5） .CONCLUSIONS： This method is simple, accurate, reproducible and canbe used as the determination of the preparations.