高效液相色谱法测定不同产地陈皮中辛弗林的含量

Content Determination of Synephrine in Pericarpium Citri Reticulate of Different Origin by HPLC Method

目的建立陈皮药材中辛弗林的含量测定方法，为更好地评价陈皮药材质量提供依据。方法采用高效液相色谱（HPLC）法，以Kromasil C18柱（4．6mm×250mm，5μm）为色谱柱；流动相：甲醇-水（水中含0．02％磷酸及0．1％十二烷基磺酸钠）（5：2）；检测波长：224nm；流速：0．6ml／min；柱温：室温。结果辛弗林在0．025—0．225μg范围内呈良好的线性关系，r=0．9998，平均加样回收率为100．15％，相对标准偏差值（RSD）=1．43％（n=6）。不同产地陈皮中辛弗林含量存在差异。结论该方法简便、快速、准确、重复性好，可用于不同产地陈皮药材的辛弗林含量测定及质量评价。

Objective To establish a method for determining the content of synephrine in pericarpium citri reticulate, and to provide basis for the quality control of pericarpium citri reticulate. Methods The HPLC method was adopted, the separation was performed on a Kromasil Clscolumn （250 mm ×4. 6 mm, 5μm）, methanol - water （0. 1% SDS and 0. 02% Phosphate is added） （5： 2） was used as the mobile phase, the detective wavelength was at 224 nm, the flow rate was 0. 6 ml/min, and the column temperature was at room temperature. Results The hesperidin showed a good linearity at the range of 0. 025 - 0. 225μg, r = 0. 99984, the average recovery was 100. 15% , RSD = 1.43% （n = 6）. The contentsof synephrine in pericarpium citri reticulate were varied from different origin. Conclusion The method is simple, quick, accurate, and has good reproducibility, it can be used to determine synephrine in pericarpium citri reticulatefrom different origin and to estimate the quality of them.