核磁共振碳谱对昆布多糖硫酸酯的结构分析

Structural Analysis on Preparation of LAMS from Laminarin by ^(13) C NMR

目的昆布多糖进行硫酸酯化后,对产物结构进行分析,为研究其生物活性奠定物质基础。方法利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,利用核磁共振碳谱研究其结构。结果13C-NMR谱显示多糖的2位羟基为SO42-的主要取代位置。结论利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,昆布多糖的2位羟基为SO42-的主要取代位置。

Objectives To sulfate the laminarin, then analyze the structure of polysaccharide sulfate, so as to make a foundation for the further research about its biological activity. Methods LAMS was prepared by chlorsulfonic acid - pyridine method ,and the structure of was analyzed by ^13 C NMR. Results The most of the SO4^2- of LAMS were on 2-OH by ^13C -NMR. Conclusion LAMS was prepared from laminarin by chlorsulfonic acid - pyridine method. The most of the SO4^2- of LAMS were on 2-OH by ^13C -NMR.