cDNA-AFLP技术及其在植物基因表达分析中的应用

cDNA-AFLP and Its Application about Gene Expressions Analysis of Plants

cDNA-AFLP是一种新的mRNA指纹图谱技术,具有重现性高、准确、可靠的特点,可对生物体转录组进行全面分析,广泛应用于基因表达特性研究、植物遗传标记分析和分离植物基因等方面。近年来随着该技术的不断发展和应用,在研究中取得了很多成果。本研究就cDNA-AFLP技术的原理和特点,在植物基因表达研究中的应用现状和发展前景进行了综述。

cDNA-AFLP is a new technique of fingerprinting of mRNA, and its high reproducibility, accuracy and reli- ability, so that it comprehensively analyse organism transcriptome, this technique is widely applied in gene expression re- search about analyzing differential expression, genetic marker analysis and identifying genes. With development and apply of this technique, many achievement were obtain in research. The paper summarizes the principle, technical features, prospect and appropriate present situation of the technique.