摘要
大肠杆菌不耐热性肠毒素(LT)具有免疫增强作用,是良好的免疫佐剂。为了更好的研究其免疫增强效果,本研究将LT的两个亚基LTa(R210G)和LTb克隆到pGST中,原核表达LTa(R210G)和LTb,经Western-blotting分析,表达蛋白具有免疫原性。对表达蛋白进行大量纯化后,以卵清蛋白为模式蛋白(OVA)为模式蛋白,免疫小鼠,ELISA结果表明,OVA+LTa(R210G)佐剂组小鼠的抗体滴度最高,OVA+LTb和OVA+LTa+LT组抗体滴度相似,高于OVA+氢氧化铝组,而OVA+氢氧化铝组高于无佐剂OVA组。因此,LTa(R210G)和LTb具有良好的免疫增强作用,是一种极有希望的免疫佐剂。
Ecoli heat-labile enterotoxin is a best immune adjuvant for its immunic enhancement. In order to study the effect of its immunic enhaneement,pGST-LTa（R210G） and pGST-LTb was constructed and expressed,the result of west- era-blotting showed the recombinant protein of GST-LTa（R21OG） and GST-LTb had good biological activities analogy to LT positive sera from pigs. We immunized mice with Ovalbumin,using GST-LTa（R210G） and GST-LTb as immunoadju- vant, and aluminum hydroxide adjuvant as controls. GST-LTa（R210G） showed the capability to remarkably increase the level of antibody against ovalbumin when comparing with those mice without any immunoadjuvant, and which was even higher than that of aluminum hydroxide adjuvant, LTb and LTa （R210G） plus LT adjuvant. LTb and LTa （R210G） plus LT adjuvant had the same level of antibody against Ovalbumin. Therefore, LTh and LTa （R210G） can become a new type of immunoadjuvant in future.
出处
《华北农学报》
CSCD
北大核心
2009年第B08期265-269,共5页
Acta Agriculturae Boreali-Sinica
基金
浙江省科技计划项目(2006C22049)
