引阳索的质量研究

Study on Quality Control of Yinyangsuo

目的:研究引阳索的质量控制。方法:建立五味子浸膏薄层鉴别方法和HPLC法测定淫羊藿浸膏中淫羊藿苷的含量测定。采用C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-水(30∶70),检测波长为270nm。结果:薄层色谱能明显检出五味子醇甲,无阴性药材干扰。淫羊霍苷进样量在0.1838～1.838μg范围内线性关系良好,r=1,平均回收率为102.4%,RSD=0.83%。结论:定性、定量方法简便、准确、重现性好,能有效控制引阳索的内在质量。

Objective： To study on quality control of Yinyangsuo. Method： Extractum Schisandra chinensis were identified by TLC. Determination of Ieariin in Extractum Epimediun by HPLC. Column is Column C18 （4. 6 mm × 250 mm,5 μm） and a mixture of methanolwater（30： 70） as the mobile phase. The detection wavelength was at 270 nm. Results： Accurately schisandrin were identified. A linear relationship of Icariin was good within 0. 183 8 - 1. 838 μg（ r = 1 ）. The average recovery was 102.4% with RSD 0. 83% （ n = 6 ）. Conclusion：The method is simple, accurate, reproducible and can be used for the quality control of Yinyangsuo.