保胎灵定性定量方法研究

Study on the Qualitative and Quantitative Methods of Baotai Ling

目的:建立保胎灵的定性定量方法。方法:采用TLC法对保胎灵中的续断、白芍和枸杞子进行鉴别;采用高效液相色谱法C18色谱柱(4.6mm×250mm,5μm);流动相为乙腈-水(10∶90);检测波长为230nm;柱温为40℃;流速为1.0mL·min-1,对保胎灵进行含量测定。结果:薄层斑点清晰,专属性强;芍药苷进样量在0.1572～0.7860μg范围内呈良好的线性关系,回归方程Y=98073.5000X-17548.2000(r=0.9998),平均回收率为100.04%,RSD为0.7%。结论:本方法简便、快速、灵敏、准确,重现性好,可有效控制保胎灵的质量。

Objective： To establish the qualitative and quantitative methods of Baotai Ling. Method： Radix dipsali and Paeony and Medlar were identified by TLC, and Paeoniflorin content in Paeonia was determined by HPLC. Symmetry C18 column（4. 6 mm× 250 mm, 5 μm） was uesd and column temperature was set at 40 ℃. The mobile phase consisted of Acetonitrile-Water（ 10： 90） ,with flow rate of 1.0 mL · min^-1. The UV detection wavelength was set at 230 nm. Results：The TLC identification was highly specific and spots were clear. Pseonifiorin showed a good linear relationship in the range of O. 157 2 -0. 786 0 μg, one-dimensional linear regression equation： Y =98 073. 500 0X - 17 548. 200 0（r =0. 999 8） ;The average recovery （n =6） was 100. 04% with RSD 1.7%. Conclusion：The method is simple, rapid, sensitive, accurate and has a good repeatability, and can be used for the quality control of Baotai Ling.