组织蛋白酶B在非小细胞肺癌中的表达及临床意义

Expression and clinical value of cathepsin B in non-small cell lung cancer

目的探讨非小细胞肺癌(NSCLC)中组织蛋白酶B(CatB)的表达及其与临床病理之间的关系。方法应用RT-PCR法检测30例NSCLC及自身配对非癌组织中CatBmRNA表达,并通过凝胶图像系统分析其相对表达量。采用免疫组化方法分析癌组织中CatB蛋白的定位和表达。结果癌组织CatBmRNA蛋白表达率为73.3%(22/30),非癌组织为13.3%(4/30)(P<0.01);癌组织CatB蛋白多为强表达,其表达率与TNM分期及淋巴结转移相关。结论NSCLC中CatB基因及蛋白均呈高表达,与临床预后密切相关。

Objective To investigate the relationship between expression of cathepsin B （CatB） and clinical pathology in non-small cell lung cancer （NSCLC）. Methods The expression of CatB mRNA in 30 NSCLC and non-cancerous lung tissues were detected by RT-PCR and the relative amount of CatB was analyzed with gel scan system. The expression and cellular distribution of CatB in the lung tissues were measured by immunohistochemistry. Results The expression rate of CatB mRNA in NSCLC was 73.3% （22/30）, which was higher than that in normal tissues （13.3%, 4/30） （P〈0. 01）. The expression level of CatB protein in NSCLC tissues was stronger and the expression rate was correlated with TNM staging and lymphatic metastasis. Conclusion There is a higher expression of CatB gene and protein in NSCLC tissues, which is associated with clinical prognosis.