粉防己碱和环孢素A联合应用延长大鼠移植心存活时间

Tetrandrine in combination with CsA prolonged survival of rat cardiac allograft

目的:探讨粉防己碱(Tet)对大鼠心脏移植的抗排斥作用。方法:分离出大鼠外周血单个核细胞(PBMC),用刀豆蛋白A(ConA)和混合淋巴细胞培养(MLC)激活单个核细胞增殖,采用四甲基偶氮唑盐(MTT)比色法法检测单纯应用粉防己碱或粉防己碱+环孢素A(CsA)联合应用后对单个核细胞增殖作用的影响;以W istar大鼠为供者,SD大鼠为受者,建立改良的颈部异位心脏移植模型,根据术后用药不同将其分为4组。①对照组:生理盐水2m l ig(灌胃);②Tet组:每日给予Tet 25 mg/kg ig;③CsA组:每日给予CsA 2 mg/kg ig;④Tet+CsA组:每日给予Tet 25mg/kg+CsA 2 mg/kg ig,观察各组移植心存活时间及病理学改变。结果:①粉防己碱能够显著的抑制单个核细胞增殖,并与CsA有协同作用。②CsA联合粉防己碱显著延长移植物的存活时间,与其它3组比较,差异均有统计学意义(P<0.01)。结论:粉防己碱能够显著的抑制单个核细胞的增殖;粉防己碱联合小剂量环孢素A能明显延长移植心存活时间。

Objective：To investigate the effects of tetrandrine on rejection of allografts of rat heart transplantation.Methods：PBMC was separated from blood of rats,and then actived by ConA,and Mixed lymphocyte culture（MLC）.MTT colorimetric assays were used to analyse proliferation of PBMC with tetrandrine or/and CsA;Cervical heart transplants were performed in the disparate rat strain combinations of Wistar donor to SD recipient.The transplanted rats were divided into 4 groups according to different administrations： group 1 ： receiving saline 2ml ig;group 2： receiving tetrandrine 25 rag/（kgod） ig; group 3 ： receiving CsA 2 rag/（ kgod ） ig; group 4 ： receiving tetrandrine 25 rag/（kgod） and CsA 2 rag/（kgod） ig. Survival of grafts and histological change were observed. Results：Tetrandrine can greatly inhibit the proliferation of PBMC in dose-dependent manner, and synergistic effects of tetrandrine and CsA were found on PBMC. The combination of tetrandrine and CsA can prolong graft survival significantly （ group 4 vs group 1,2,3 ） （P 〈 0.01 ）. Conclusion ：Tetrandrine can inhibit the proliferation of PBMC ; Tetrandrine has a synergistic effect with subtherapeutic doses of CsA in prolonging rat cardiac allograft survival.