马齿苋甜菜红素对衰老小鼠抗氧化系统的影响

Effect of betacyanins extracted from Portulaca oleracea L.on the antioxidation system in senile mice

目的:研究马齿苋(Portulaca oleracea L.)甜菜红素对衰老模型小鼠抗氧化性能的影响。方法:小鼠颈背部sc 5%D-半乳糖1mg/kg.d 7周制备衰老模型,维生素E或马齿苋甜菜红素100、200及400 mg/kg.d ig给予,每日1次,给药7周。分别测定小鼠脑组织的脂褐质含量,心、肝、肾中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果:和正常组比较,衰老模型组小鼠脑脂褐质含量明显增加,心、肝、肾组织中MDA含量升高,SOD及GSH-Px活力下降。维生素E和马齿苋甜菜红素灌喂显著降低衰老小鼠脑脂褐质含量和心、肝、肾组织中MDA含量,提高心、肝、肾组织中SOD及GSH-Px活力,量效关系均呈正相关。马齿苋甜菜红素抗氧化效果明显优于同剂量维生素E。结论:马齿苋甜菜红素可以改善衰老小鼠的抗氧化性能。

Objective： This experiment was designed to explore the effect of betacyanins extracted from Portulaca oleracea L on the antioxidant ability in senile mice.Methods： Kunming mice were randomly divided into 8 groups including control,senile mode,100,200 and 400 mg/kg·d vitamin E and betacyanins extracted from Portulaca oleracea L.,10 mice in each group.Mice were injected sc D-galactose 1 mg/kg·d for 7 weeks to obtain senile mode.At the same time,100,200 and 400 mg/kg·d vitamin E or betacyanins were given ig for 7 weeks. Lipofuscin（LF） content in brain, and malondialdehyde （MDA） content, superoxide dismutase （SOD） and glntathione peroxidase （GSH-Px） activities in heart, liver and kidney were measured respectively. Results： Compared with control group, LF content in brain significantly increased, MDA content markedly elevated while SOD and GSH-Px activities notablely dropped in heart, liver and kidney of mice in senile model group. Betacyanins and vitamin E dose-dpendently reduced LF content in brain, decreased MDA content while increased SOD and GSH-Px activities in heart, liver and kidney of senile mice, but the effects of betacyanins were more notable than vitamin E. Coneluslon： The effects of betaeyanins were much better than vitamin E in strenztheninz the activities of antioxidases in senile mice.