三羟异黄酮降低去卵巢大鼠外周血炎性因子和动脉张力

Effects of Genistein on Serum Inflammatory Factors Level and Tension of Aorta Ring in Ovariectomized Rats

目的已有实验证实三羟异黄酮(GST)对绝经后妇女具有较好的心血管保护作用。观察GST对去卵巢大鼠外周血白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和核转录因子κB(NF-κB)表达以及主动脉张力变化的影响,旨在探讨GST对绝经后女性心血管保护作用的机制。方法将40只成年雌性大鼠随机分成4组:假手术组(n=10),去卵巢组(n=10),雌激素组[20μg/(kg.d),n=10],GST组[200μg/(kg.d),n=10]。第8周末取标本,酶联免疫法测定大鼠血清IL-6、TNF-α和NF-κB水平。结果去卵巢组炎症标志物水平显著高于对照组[IL-6(95.8±16.2)比对照组(40.2±2.8)ng/L;TNF-α(60.3±6.4)比对照组(18.3±3.3)ng/L;NF-κB(11.9±3.3)比对照组(2.6±1.2)ng/L,均P<0.01]。补充GST后IL-6(43.4±3.7)ng/L、TNF-α(19.9±3.2)ng/L和NF-κB(2.2±1.0)ng/L水平均显著降低(均P<0.01)。去卵巢大鼠主动脉张力[最大收缩力(4.58±1.36)g]明显高于对照组[最大收缩力(1.83±0.95)g,P<0.01)],补充GST可明显降低动脉血管张力[最大收缩力(1.99±0.39)g,P<0.01]。结论GST能显著降低去卵巢大鼠外周血中IL-6、TNF-α和NF-κB水平和主动脉张力。

Objective To study the effects of genistein （GST） on the serum level of IL-6, TNF-α, NF-κB and vascular tension of ovariectomized rats, explore the cardiovascular protective mechanism of GST. Methods Forty female Wistar rats were randomized to receive the following approaches： sham-operated （control, n= 10 ）, ovariectomized （OVX, n= 10）, ovariectomized with 17β-estradiol replacement [20 μg/（kg · d）, n = 10], ovariectomized with genistein replacement [200μg/（kg · d）, n 10]. Eigth weeks after GST or 17β-E2 replacement therapy in ovariectomized （OVX） rats, ELISA method was used to determine the IL-6, TNF-α and NF-κB levels in serum. Results The serum levels of IL-6, TNF-α and NF-κB were markedly higher in the OVX group than in controls [IL-6（95.8±16.2 ） vs control （ 40.2 ± 2.8 ） ng/L ; TNF-α （ 60.3 ± 6.4 ） vs control （ 18.3 ± 3.3 ） ng/L ; NF-κB （ 11. 9 ± 3.3 ） vs control （2.6± 1.2）ng/L, all P〈0.01]. Replacement therapy prevent the increases in IL-6, TNF-α, and NF-κB in ovariectomized rats（all P〈0. 01）. The increased vascular tension induced in ovariectomized rats were also significantly decreased by GST or 17β-E2. Conclusion GST inhibit the levels of IL-6, TNF-α and NF-κB in serum of ovariectomized rats, and decrease the vascular tension in ovariectomized rats.