微模式化基底上大鼠骨髓间充质干细胞的增殖、分化和迁移

Proliferation,differentiation,and migration of rat bone marrow mesenchymal stem cells on micropatterned substrate

目的利用微模式化基底研究基底几何微结构对骨髓间充质干细胞增殖、分化及迁移过程的影响。方法设计制作微模式化基底,用以控制细胞的铺展形态和面积。比较不同模式控制下大鼠骨髓间充质干细胞的增殖、分化和迁移数据。结果细胞铺展宽度狭小可抑制骨髓间充质干细胞的增殖,细胞铺展形状可以调节其向成骨细胞分化的进程,细胞铺展面积受限时迁移增强,其增殖迁移行为减弱与成骨细胞诱导因子地塞米松的作用有关。结论细胞铺展的几何形状和面积是骨髓间充质干细胞增殖、分化及迁移过程中的重要调节因子。

Objective To quantify the micro-geometric and configuration of micropatterned substrate and analyze its effects on proliferation,differentiation,and migration of bone marrow mesenchymal stem cells（BM MSCs）.Method Micropatterned substrates were designed and fabricated to control cells spreading shape and area.Data of proliferation,differentiation and migration of rat BM MSCs on various patterned substrates were compared.Result Proliferation of BM MSCs was inhibited when their spreading width was narrowed.Osteoblastic differentiation was regulated distinctively in different spreading shape and area. Cell migration was more active when their spreading area was reduced. Dexamethasone was found to play an important role in down-regulating cell proliferation and migration on patterned substrate.Conclusions Cell spreading shape and area is important factors for re qulatinq proliferation, differentiation, and migration of BM MSCs.