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碱性磷酸酶组化染色在克罗恩病诊断中的价值 被引量：2

Value of alkaline phosphatase histochemical staining in diagnosis of Crohn’s disease
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摘要 目的探讨碱性磷酸酶组化染色在克罗恩病（CD）诊断中的价值。方法应用BCIP/NBT组化染色检测手术切除的15例克罗恩病和10例溃疡性结肠炎中碱性磷酸酶活性表达水平。结果15例克罗恩病中未受损的肠黏膜表面刷状缘呈紫蓝色BCIP/NBT为（＋）,而10例溃疡性结肠炎BCIP/NBT均（-）。结论BCIP/NBT组化染色对诊断克罗恩病具有一定的价值。肠黏膜活检中HE常规染色结合BCIP/NBT组化染色可提高CD的诊断正确率。 Objective To study the value of alkaline phosphatase histochemical staining in the diagnosis of Crohn＇s disease. Methods Fifteen cases of Crohn＇ s disease （CD）, and 10 ulcerative colitis （UC） were detected by BCIP/NBT histochemical staining. Results BCIP/NBT positive staining was observed in the brush border in unvolved areas of inflammation in CD, but no BCIP/NBT staining was detected in UC. Conclusion BCIP/NBT alkaline phosphatase histochemical staining may be useful in the dangnosis of CD. The combination of morphology with the histochemistry may improve the correct diagnostic rate of intestinal mucosa biopsy.
作者 王劲松 黄文斌 李俐 张丽华 朱晓群 潘敏鸿
机构地区 南京医科大学附属南京第一医院病理科 南京市鼓楼医院病理科 皖南医学院病理学教研室 南京军区南京总医院病理科
出处 《诊断病理学杂志》 CSCD 2009年第4期258-260,共3页 Chinese Journal of Diagnostic Pathology
关键词 克罗恩病 溃疡性结肠炎 BCIP/NBT 组化染色 Alkaline phosphatase BCIP/NBT Crohn s disease Ulcerative colitis Histochemistry
分类号 R574.1 [医药卫生—消化系统]
  • 相关文献

参考文献11

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共引文献7

同被引文献20

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诊断病理学杂志
2009年 第4期

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