PCR-SSCP检测急性髓系白血病患者FLT3基因及FLT3/ITD基因突变

The detection of FLT3 and FLT3/ITD gene mutation by polymerasechain reaction single-strand conformation polymophism in acute myeloid leukemia patients

目的研究急性髓系白血病(AML)患者DNA水平FLT3基因及其内部串联重复(ITD)突变。方法采用多聚酶链反应(PCR)联合单链构象多态性(SSCP)方法检测43例不同免疫分型AML患者DNA水平FLT3基因及FLT3/ITD基因突变。结果43例AML患者经PCR扩增,琼脂糖凝胶电泳FLT3基因检测全部阳性,未发现FLT3/ITD基因突变;SSCP电泳FLT3基因检测全部阳性,43例AML患者中有15例(35%)出现FLT3/ITD基因突变。结论PCR-SSCP适用于检测急性髓系白血病FLT3/ITD基因突变分析。

Objective To study the FLT3 gene expression and its internal tandem duplication （ ITD）mutation in acute myeloid leukemia （AML） patients.Methods Polymerase chain reaction （PCR）and single strand conformation polymophism （SSCP） were used to detect FLT3 gene and FL T3/1TD mutation in 43 AML patients. Results The expression of FL 33 gene were detected in 43 AML patients with agarose gel and single-strand conformation polymorphism eletrophoresis;There was no FLT3/ITD mutation withagamse gel eletrophoresis, but FLT3/1TD mutation was detected in 15 cases （35%）with single-strand conformation polymorphism gel eletrophoresis. Condttsion PCR-SSCP was suitable to detected FLT3/ITD mutation in acute myeloid leukemia （AML） patients.