邻联茴香胺-H_2O_2-辣根过氧化物酶伏安酶联免疫分析检测四种重要植物病毒

Detection of Four Important Plant Viruses with Voltammetric Enzyme Linked Immunoassay Based on ODA H 2O 2 HRP System

将邻联茴香胺（ＯＤＡ）Ｈ２Ｏ２辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析体系应用于测定南芥菜花叶病毒（ＡｒａｂｉｃＭｏｓａｉｃＶｉｒｕｓＡｒＭＶ）、黄瓜花叶病毒（ＣｕｃｕｍｂｅｒＭｏｓａｉｃＶｉｒｕｓＣＭＶ）、南方菜豆花叶病毒（ＳｏｕｔｈｅｒｎＢｅａｎＭｏｓａｉｃＶｉｒｕｓＳＢＭＶ）和芜菁花叶病毒（ＴｕｒｎｉｐＭｏｓａｉｃＶｉｒｕｓＴｕＭＶ）等四种重要植物病毒，其灵敏度高于酶联免疫吸附显色光度法（ＥＬＩＳＡ）。

Arabic mosaic virus(ArMV),cucumber mosaic virus(CMV),southern bean mosaic virus(SBMV)and turnip mosaic virus(TuMV) have been detected by voltammetric enzyme linked immunoassay based on o dianisidine(ODA) H 2O 2 horseradish peroxidase(HRP) system.The highest dilution ratios to detect the leaf saps infected by ARMV,CMV,SBMV and TuMV were :1∶1×10 5,1∶2.5×10 4,1∶2.5×10 6 and 1∶5×10 5,and the detection ranges 1∶10～1∶1×10 5,1∶10～1∶2.5×10 4,1∶5×10 2～1∶2.5×10 6and 1∶2.5×10 3～1∶5×10 5,respectively. The sensitivity of the presented method was higher than that of the enzyme linked immunosorbent assay(ELISA) method.