联苯胺-H_2O_2-HRP伏安酶联免疫分析新体系酶催化反应机理的研究

Enzyme Catalyzed Reaction in New Benzidine H 2O 2 HRP Voltammetric Enzyme Linked Immunoassay System

首次利用红外光谱法、紫外可见光谱法对联苯胺Ｈ２Ｏ２辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析新体系酶催化反应产物进行了鉴定。结果表明，在ｐＨ４条件下，ＨＲＰ能催化Ｈ２Ｏ２氧化联苯胺生成偶氮联苯胺，提出了相应的反应机理。

The product of horseradish peroxidas(HRP) catalyzed H 2O 2 oxidizing benzidine was investigated with infrared spectroscopy and UV Vis spectorscopy,The mechanism of reaction is made clear. It indicates that HRP catalyses the reaction of H 2O 2 oxidizing benzidine to produce bisazobiphenyle if the enzyme catalyzed reaction is carried out in pH4.