RP-HPLC同时测定银翘解毒片中绿原酸、连翘苷和甘草酸的含量

Quantitative determination of chlorogenic acid,forsythin and glycyrrhizic acid in Yinqiaojiedu Tablets by RP-HPLC simultaneously

目的研究银翘解毒片中绿原酸、连翘苷和甘草酸的含量和测定方法。方法采用反相高效液相色谱法，色谱柱为Diamonsil^TM C18（250mm×4．6mm，5μm），乙腈-0．1％磷酸水溶液为流动相，梯度洗脱，流速为1．0mL／min，三波长检测，λ326nm，λ2为228nm，λ3为252nm，柱温30℃。结果绿原酸、连翘苷和甘草酸进样量分别在0．3956～2．7692μg、0．5928—4．1496μg和0．5583—3．9080μg范围内呈良好的线性关系，相关系数分别为0．9991、0．9998和0．9999，平均加样回收率分别为100．5％、97．1％和99．6％，RSD分别为1．6％、2．1％和1．2％（n=6）。结论该方法专属性好、准确度高，可用于综合评价银翘解毒片的质量。

Objective To study the contents and determination method of chlorogenic acid, forsythin and glycyrrhizic acid in Yinqiaojiedu Tablets. Methods The procedure of RP-HPLC was performed on a column of Diamonsil^TM C18 （250 mm ×4.6 mm, 5 μm） using acetonitrile-0.1% phosphoric acid water solution as the mobile phase in gradient elution. The flow rate was 1.0 mL/min,λ1 was 326 nm, λ2 was 228 nm, and λ3 was 252 nm in three-wavelength detection. The column temperature was 30℃. Results The sample size of chlorogenic acid, forsythin and glycyrrhizic acid showed respectively a good linear relationship at range of 0.395 6 -2.769 2 μg, 0.592 8 -4. 149 6 μg and 0.558 3 -3.908 0 μg （r= 0.999 1, r=0.999 8 and r=0.999 9）. The average recovery （n=6） was 100.5%, 97. 1% and 99.6% （RSD = 1.6%, 2. 1% and 1.2% ） respectively. Conclusion The method is peculiar with a good reproducibility, which can be used for the qualitative evaluation of Yinqiaojiedu Tablets.