高效液相色谱法-蒸发光散射检测器测定安神胶囊中酸枣仁皂苷A和B的含量

Determination of Jujuboside A and B in Anshen Capsules with HPLC-ELSD

目的建立安神胶囊中酸枣仁皂苷A和B含量的测定方法。方法应用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD),以YMC-PackODS柱(200mm×4.6mm,5μm)为色谱柱,甲醇-水-冰醋酸(70∶30∶2)为流动相,流速是1.0mL/min,柱温:25℃;漂移管温度95℃,气体流速1.05L/min。结果酸枣仁皂苷A线性范围是2.47～12.34μg,r=0.9999,平均回收率为99.3%,RSD=1.89%(n=6);酸枣仁皂苷B线性范围是1.34～6.70μg,r=0.9999,平均回收率为99.3%,RSD=1.5%(n=6)。结论该方法快速简便,重现性良好,可用于测定安神胶囊中酸枣仁皂苷A和B的含量。

Objective To establish a method for the determination of Jujubaside A and B in Anshen Capsules. Method A HPLC-ELSD was used. The chromatography column was YMC-Pack ODS （200mm×4.6mm, 5μm）. The mobile phase was consisted of methanol-water-attic acid （70 ： 30 ： 2） with flow rate of 1.0mL/min, and the column temperature was kept at 25 ℃ .The temperature of drift tube was 95℃ and the gas flow rate was 1.05 L/min. Result The linear relationship was good. The amount ofjujuboside A was within the range of 2.47-12.34μg（r=0.9999）, and the average recovery was 99.3% （RSD=1.9%, n=6）. The amount ofjujuboside B was within the range of 1.34-6.70μg （r=0.9999）, and the average recovery was 99.3% （RSD=1.5%, n=6）. Conclusion The method was shown to be suitable for the determination of the content of jujuboside A and B in Anshen Capsules.