摘要
目的探讨肠易激综合征（irritable bowel syndrome）的发病机制以及痛泻要方煎剂治疗肠易激综合征内脏高敏感性模型大鼠的机制。方法选用清洁级新生SD大鼠，采用结肠慢性刺激法加夹尾刺激法造模。造模成功后，将实验动物分组为空白对照组（A组）、模型对照组（B组）、中药低剂量组（c组）、中药中剂量组（D组）、中药高剂量组（E组）、西药奥替溴铵（F组）。A组和B组以生理盐水按4ml／100g（40g／kg／d）灌胃，C、D、E、F组分别以低剂量痛泻要方煎剂组按0．4ml／100g（4g／kg／d）、中剂量痛泻要方煎剂组按1．2ml／100g（12g／kg／d）、高剂量痛泻要方煎剂组按4ml／100g（40g／kg／d）灌胃、西药奥替溴铵组按4ml／100g（150g／kg／d）灌胃，每天两次，连续30天。结果模型大鼠血清sP含量明显增加，痛泻要方高剂量组与西药组斯巴敏都能使模型大鼠血清sP含量明显回落，且中药高剂量组与斯巴敏组间无差异。模型大鼠结肠粘膜sP含量明显增加，痛泻要方中、高剂量组与西药组斯巴敏都能使模型大鼠结肠粘膜sP含量明显回落，且中药中、高剂量组与斯巴敏组间无差异。结论痛泻要方的作用机制可能是通过降低模型大鼠血浆和结肠组织sP含量来实现的。
Objective： To explore the pathogenesis of irritable bowel syndro--me （IBS） and the mechanism of curing experimental rat model of IBS in Tongxieyaofang decoction. Methods： Model rats of IBS, which adopt the model chronic rectal irritation and stimulation with forceps clip on tails in the neonatal rats. On the base of above successful model, the rates were randomly divided into six groups： the normal control group （Group A）, the model group （Group B）, the low dosage of Tongxie Yaofang decoction （Group C）, the moderate dosage of Tongxie Yaofang decoction （Group D）, the high dosage ofTongxie Yaofang decoction （Group E）, the Spasmomen control group （Group F） .Group A and Group B received oral administration in 4ml/100g （40g/kg/d） of NS, Group C received oral administration in 0.4ml/100g/ （4g/kg/d）, Group D received oral administration in 1.2ml/100g （12g/kg/d）, Group E received oral administration in 4ml/100g （40g/kg/d）, Group F received oral administration in 4ml/100g （40g/kg/d） twice everyday for one month. Results： The level of substance P in plasma of the model group rats increased significantly.The high dosage of Tongxieyaofang group and the group of Sibamin were able to make the level of substance P descends signify-cantly.And there is no difference between the high dosage of Tongxieyaofang group and the group of Sibamin.The level of substance P in mucosa of bowel of the model group rats increased significantly. The high dosage of Tongxieyaofang group , the middle dosage of Tongxieyaofang group and the group of Sibamin were able to make the level of substance P in mucosa of bowel descend significantly. And there is no difference in the three groups.Conclusion： The possible mechanism of Tongxieyaofang ： It makes substance P in plasma and mucosa descends significantly.
