缺氧复合NaCN中毒对大鼠心肌线粒体氧化应激指标的影响

Effects of combined hypoxia and sodium cyanide intoxication on oxidative stress parameters in cardiac muscle mitochondria of rats

目的:观察缺氧复合氰化钠(NaCN)中毒对大鼠心肌线粒体氧化应激反应的影响。方法:40只雄性SD大鼠随机均分为平原对照、平原中毒、高原对照、高原中毒组。高原组动物在模拟海拔4000 m的低压舱内适应3 d后开始实验。对照组动物皮下注射生理盐水。中毒组皮下注射3.6 mg/kg NaCN,分别于0.5、1、2 h时点处死动物,取心肌制备线粒体。检测线粒体羟自由基(OH.)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果:缺氧复合NaCN中毒致心肌细胞线粒体OH.、MDA含量显著升高,SOD活性显著降低。结论:相较于单纯模拟缺氧、单纯NaCN中毒,缺氧复合NaCN中毒会加重心肌线粒体的损伤。

Objective： To observe the effects of combined hypoxia and sodium cyanide （NaCN） intoxication on oxidative stress reactions in cardiac muscle mitochondfia of rats. Methods ：40 male SD rats were randomly divided into plain control group,NaCN intoxicated group at 308 m altitude and high altitude control group, NaCN intoxicated group at simulated high altitude of 4000 m. Animals in two high altitude groups were scheduled to stay for 3 days in a decompression chamber till the test began. Two control groups were injected with physiological saline and intoxicated groups were injected subcutaneously with NaCN at 3.6 mg/kg. Animals were killed at 0. 5,1 and 2 hours after treatment and the mitoehondria samples were prepared from the cardiac muscle tissues. The contents of OH · and MDA as well as the SOD activity were determined by spectrephotometrie method. Results： It was demonstrated that hypoxia and NaCN injection could significantly increase the contents of OH · and MDA while decreasing the SOD activity in mitochondrial samples of rats. Conclusion： Compared with simple NaCN intoxication or simple hypobarie hypoxia, the combination of acute hypobaric hypoxia and NaCN intoxication may aggravate the cardiac muscle cytochondfiome injury.