鸡败血霉形体SC克隆致弱株免疫原性试验

Immunogenecity Detection of Attenuated Mycoplasma gallisepticum Strain SC

在透明塑料隔离器中进行了鸡败血霉形体人工发病试验，结果３０日龄ＳＰＦ来航鸡在ＮＤＶ、ＩＢＶ弱毒诱导和７０／１００００００（质量分数）氨环境刺激的联合作用下，致病率可达１００％（７／７）。经鸡败血霉形体Ｓ６克隆致弱株ＳＣＦ１５６代培养物点眼、滴鼻免疫后１０，２０，３０ｄ的ＳＰＦ来航鸡再以ＭＧ强毒攻毒，结果，保护率分别达８０．０％（４／５），７５．０％（３／４）和８７．５％（７／８）。近期免疫效果证明，鸡败血霉形体Ｓ６克隆致弱株ＳＣ有良好的免疫原性。

A model of artificial infection of Mycoplasma gallisepticum (MG) was established in isolators. 30 day old SPF Leghorn chicken stressed by inoculation of attenuated NDV, IBV and challenged with MG in an atmosphere containing 70/1 000 000 ammonia showed 100% (7/7) airsacculitis. SPF Leghorn chicken inoculated by aerosol and ocular instillation with attenuated MG cloned strain SC were challenged respectively with virulent strain 10, 20 and 30 d postinoculation, and 80.0%(4/5), 75.0%(3/4), 87.5%(7/8) of chickens were protected from the airsacculitis, while 100% of control chickens developed typical airsacculitis. These initial results indicated that MG cloned avirulent strain SC could effectively produce the protective immunity 10 to 30 days postinoculation.