疏肝方、健脾方、疏肝健脾方对肝郁脾虚证模型大鼠IL-2、IL-6及T淋巴细胞增殖率的影响

Influences of formulas with actions of soothing liver and invigorating spleen on IL-1,IL-6 and proliferation rate of T lymphocytes in rat model of liver-stagnation and spleen-deficiency syndrome

目的比较疏肝、健脾、疏肝健脾方对肝郁脾虚证模型大鼠免疫系统的影响。方法大鼠随机分为正常对照组、肝郁脾虚证组、柴胡疏肝散组、四君子汤组、柴疏四君汤组5组,每组12只。后4组大鼠采用慢性束缚+过度疲劳+饮食失节法,连续4周造模,正常对照组不予处理。其中3个中药干预方组分别于造模第2周末,按10 mL/g给予相应方药灌胃,连续2周;模型组和正常对照组给予等量蒸馏水。于实验第29天,处杀各组大鼠,测定血清IL-2、IL-6及脾T淋巴细胞增殖率。结果与正常组相比,模型组大鼠血清IL-2和IL-6及脾T淋巴细胞增殖率均见显著性降低(P<0.05)。与模型组相比,柴胡疏肝散组、四君子汤组、柴疏四君汤组3组大鼠血清IL-2均呈显著性升高(P<0.05);柴胡疏肝散组和柴疏四君汤组血清IL-6呈显著性升高(P<0.05);柴疏四君汤组脾T淋巴细胞增殖率呈显著性升高(P<0.05)。结论疏肝方、健脾方、疏肝健脾方对肝郁脾虚证模型的免疫低下均有不同程度的改善作用,但以柴疏四君汤的作用最佳;疏肝健脾方与肝郁脾虚证之间具有更高的关联性。

Objective To compare the influences of formulas with actions of soothing liver, invigorating spleen or soothing liver and invigorating spleen on immune system in rat model of liver-stagnation and spleen-deficiency syndrome. Method All rats were randomly divided into the normal control group （ group 1 ）, group of liver-stagnation and spleen-deficiency syndrome （ group 2）, Chaihushugan Powder group （ group 3 ） , Sijunzi Decoction group （ group 4） and Chaishusijun Decoction group （ group 5, each n = 12）. The model was established in group 2, 3, 4 and 5 by chronic restraint-fatigue-diet disorder method for 4 weeks. Group 3, 4 and 5 were given orally the relevant formulas （ 10 mL/g） for 2 weeks at the end of 2nd week, and group 1 and 2 were given equivalent distilled water. On the 29th day all rats were killed and serum interleukin-2 （IL-2） , interleukin-6 （IL-6） and proliferation rate of T lymphocytes were detected. Result Compared with group 1, serum IL-2, IL-6 and proliferation rate of T lymphocytes decreased significantly in group 2 （P 〈0. 05 ）. Compared with group 2, serum IL-2 increased significantly in group 3, 4 and 5 （ P 〈0. 05 ）, serum IL-6 increased significantly in group 3 and 5 （ P 〈 0.05 ）, and proliferation rate of T lymphocytes increased significantly in group 5 （P 〈 0. 05 ）. Conclusion Formulas with actions of soothing liver, invigorating spleen or soothing liver and invigorating spleen can relieve the hypoimmune state of the model of liver-stagnation and spleendeficiency syndrome, among them Chzishusijun Decoction is the best. Formulas with actions of soothing liver and invigorating spleen is related more closely to liver-stagnation and spleen-deficiency syndrome.