HPLC法测定苦参药材中苦参酮、降苦参酮的含量

Quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescentis by HPLC

目的建立测定苦参药材中2种黄酮类成分苦参酮和降苦参酮含量的高效液相色谱方法。方法采用PhenomenexGemini C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水（50∶50）为流动相,流速1.0 mL/min,检测波长290 nm。结果苦参酮和降苦参酮的线性范围分别为0.318 6-1.593 0μg（r=0.999 8）,0.112 8~0.564 0μg（r=0.999 9）;苦参药材平均加样回收率（n=6）分别为100.56%（RSD=0.93%）,96.66%（RSD=0.85%）。结论3批样品测定结果表明,该方法简便、准确、重现性良好,可用于苦参药材苦参酮和降苦参酮2种黄酮类成分的含量测定。

Objective To establish a method for quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescerttis by HPLC. Methods HPLC was performed on a column of Phenomenex Gemini C18（250 mm ×4.6 mm, 5 um）, and using acetonitrile-water （50： 50） as the mobile phase. The flow rate was 1.0 mL/min and detection wavelength was 290 nm. Results Kurarinone showed a good linear relationship at range of 0.3186 - 1. 5930 ug （ r =0. 999 8） and norkurarnone, at range of 0.1128 - 0.5640 ug （r = 0. 999 9 ）. The average recovery （ n = 6） of kurarinone was 100.56% （ RSD = 0.93% ）, and norkurarnone, 96.66% （ RSD = 0.85% ）. Conclusion The determinative results of 3 batches of samples showed that the method is easy and accurate with a higher reproducibility, and it can be used in the quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescentis.