摘要
目的建立测定苦参药材中2种黄酮类成分苦参酮和降苦参酮含量的高效液相色谱方法。方法采用PhenomenexGemini C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(50∶50)为流动相,流速1.0 mL/min,检测波长290 nm。结果苦参酮和降苦参酮的线性范围分别为0.318 6-1.593 0μg(r=0.999 8),0.112 8~0.564 0μg(r=0.999 9);苦参药材平均加样回收率(n=6)分别为100.56%(RSD=0.93%),96.66%(RSD=0.85%)。结论3批样品测定结果表明,该方法简便、准确、重现性良好,可用于苦参药材苦参酮和降苦参酮2种黄酮类成分的含量测定。
Objective To establish a method for quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescerttis by HPLC. Methods HPLC was performed on a column of Phenomenex Gemini C18(250 mm ×4.6 mm, 5 um), and using acetonitrile-water (50: 50) as the mobile phase. The flow rate was 1.0 mL/min and detection wavelength was 290 nm. Results Kurarinone showed a good linear relationship at range of 0.3186 - 1. 5930 ug ( r =0. 999 8) and norkurarnone, at range of 0.1128 - 0.5640 ug (r = 0. 999 9 ). The average recovery ( n = 6) of kurarinone was 100.56% ( RSD = 0.93% ), and norkurarnone, 96.66% ( RSD = 0.85% ). Conclusion The determinative results of 3 batches of samples showed that the method is easy and accurate with a higher reproducibility, and it can be used in the quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescentis.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第6期410-412,418,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.30772710)
关键词
苦参
苦参酮
降苦参酮
高效液相色谱法
含量测定
Radix Sophorae Flavescerttis
kurarinone
norkurarnone
HPLC
quantitative determination