针刺对脑挫伤大鼠脑组织P^53、Bcl-2、Bax表达的影响

Effects of acupuncture on expressions of brain P^(53),Bcl-2 and Bax in rats with brain contusion

目的观察针刺对脑挫伤模型大鼠脑组织凋亡相关基因P53、Bcl-2、Bax表达的影响。方法参照Feeney自由落体冲击造模法建立脑挫伤大鼠模型,将40只大鼠随机分为假手术组、模型组、纳络酮组、针刺组,每组10只。针刺组、纳络酮组分别给予针刺、腹腔注射纳络酮治疗,每天1次,共治疗7 d。处理结束后,采用免疫组织化学方法检测损伤脑组织P53、Bcl-2/Bax表达。结果模型组大鼠P53、Bax表达较假手术组明显升高(P<0.01),Bcl-2/Bax比值下降;针刺组、纳络酮组与模型组比较:Bcl-2表达明显升高(P<0.01),P53、Bax表达明显下降(P<0.01),Bcl-2/Bax比值升高。针刺组与纳络酮组P53、Bcl-2、Bax表达无明显差异(P>0.05)。结论针刺可减少脑挫伤大鼠脑组织促凋亡基因P53和Bax表达、增加凋亡抑制基因Bcl-2的表达,升高Bcl-2/Bax比率,这可能是针刺减少脑细胞凋亡、防治继发性脑损害的机制之一。

Objective To observe the effects of acupuncture on expressions of brain apoptosis-related genes, i.e. p53, Bcl-2 and Bax, in rats with brain contusion. Method The rat model of brain contusion was established by using impact method of Feeney freely falling body and then all rats （n = 40 ） were randomly divided into 4 groups, including the sham-operation group, model group, naloxone group and acupuncture group （each n = 10）. The acupuncture group and naloxone group were given respectively acupuncture and introperitoneal injection of noloxone once a day for 7 days. The expressions of P^53, Bcl-2 and Bax in injured brain were detected by using immunohistochemistry assay. Result In the model group the expressions of p53 and Bax were higher significantly than those in the sham-operation group （P 〈0.01 ） and the ratio of Bcl-2 to Bax was reduced. In the acupuncture group and naloxone group the expression of Bcl-2 was higher significantly （P 〈0.01 ）, expressions of p53 and Bax were lower significantly （P 〈0.01 ） than those in the model group, and the ratio of Bcl-2 to Bax was increased. The expressions of P^53, Bcl-2 and Bax were not different respectively between the acupuncture group and naloxone group （P 〉 0.05 ）. Conclusion Acupuncture can reduce the expressions of p53 and Bax, increase Bcl-2 expression, and raise the ratio of Bcl-2 to Bax in rats with brain contusion, which may be one of the mechanisms of acupuncture for relieving brain apoptosis and preventing secondary brain injury.