TIMP-4和MMP-2在原发性肝癌的表达及临床意义

Expression of TIMP-4 and MMP-2 in Primary Carcinomas of Liver and Its Significance

目的:探讨组织基质蛋白酶抑制剂4(tissue inhibitor metalloproteinase-4,TIMP-4)和基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)在原发性肝癌的表达及临床意义。方法:采用免疫组织化学方法检测40例不同病理分期、分级的原发性肝癌组织中TIMP-4和MMP-2的表达情况,同时取15例正常肝组织作为对照。结果:MMP-2蛋白在肿瘤组织中表达远多于正常组织,在正常组织中仅呈散在表达且表达较弱;TIMP-4蛋白在肿瘤组织中表达远多于正常组织,在正常组织中仅呈散在表达且表达较弱,在肿瘤组织中两者表达比例失调;随着原发性肝癌临床病理分期、分级的升高,MMP-2表达呈现增高趋势(P<0.05),TIMP-4表达呈现减低趋势(P<0.05)。结论:MMP-2与TIMP-4表达比例失调与原发性肝癌的浸润和转移有关。

Objective：To investigate the expression of TIMP-4 and MMP-2 in primary carcinomas of liver and its significance. Methods：The expression of TIMP-4 and MMP-2 in 40 cases of primary carcinomas of liver in various grading and staging was detected by immunohistochemical method,then compared with those in 15 normal controls. Results：The expression of TIMP-4 and MMP-2 in primary carcinomas of liver was higher than those in normal tissues,which showed weak and negative expression.The ratio of TIMP-4 to MMP-2 was disproportionate. The expression of MMP - 2 was increased but the expression of TIMP - 4 was decreased with the grading and staging of primary carcinomas of liver（ P 〈 0.05）. Conclusion： The disproportionate expression of TIMP - 4 to MMP - 2 is correlated with invasion and metastasis of primary carcinomas of liver.